Document Type : Original Article
Authors
1
Zoology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt.
2
Forensic and Toxicology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt.
3
College of Medicine, Suez Canal University
Abstract
Background: Scorpion maurus palmatus belongs to the family scor-pionidae and is common in the Saudi Aribia, Middle East, Mediterranean and Jordan regions. It is found in lower Egypt (Wadi Natrun, Cairo, Faiyum, Western Mediterranean costal Desert), southern and central Sini. Study aim: is to study the cytotoxicity induced by the scorpion venom of Scorpio maurus palmatus inhabiting different localities in Egypt using different in vitro approaches. Material and methods: Collection of scorpions and preparation of venom samples. Captive scorpions from the four locations (Alexandria,Rahaba, Sahab, and Alagramia) were kept separately in individual containers. Scorpions were milked using the squeezing method, and individual venom samples collected and lyophilized. The freeze-dried pooled venom was stored at -20 °C prior to use. Assays: Malondialehyde (MDA) assay, Protein carbonyl assay, Estimation of nitric oxide, Estimation of reduced glutathione (GSH), Cu/Zn-superoxide dismutase and catalase, Estimation of hemoglobin content. Results: The haemoglobin level was significantly (p<0.05) increased with time as revealed by ANOVA. According to Two-way analysis of variance there were a highly significant difference in glutathione level induced by groups (p<0.001***), sites (p<0.001***), concentrations (p<0.001***). The glutathione level (µg/ mL) was significantly (p<0.05) increased with time as revealed by ANOVA in Alexandria, Rahaba, Agramia, however it was decreased in Sahab. According to Two-way analysis of variance there were a highly significant difference in SODActivities induced by groups (p<0.001***), sites (p<0.001***), concentrations (p<0.001***).
Keywords
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