Histological and immunohistochemical study on the possible ameliorating effects of antox on the parotid gland of rats with Streptozotocin- Induced Diabetes Mellitus Type 1

Morsy, Manal Mohammad; Abdelwahab, Ola Ali; Ibrahim, Fatma; Hassan, Heba Ahmed; Alabassery, Nadia

doi:10.21608/esctj.2022.179739.1023

Histological and immunohistochemical study on the possible ameliorating effects of antox on the parotid gland of rats with Streptozotocin- Induced Diabetes Mellitus Type 1

Document Type : Original Article

Authors

¹ Human Anatomy & Embryology department, Faculty of Medicine, Zagazig University, Egypt.

² Anatomy and embryology department, Faculty of medicine, Zagazig University

³ Forensic medicine and clinical toxicology department, Faculty of Medicine, Zagazig University, Egypt

⁴ Clinical pharmacology departments, Faculty of Medicine, Zagazig University, Egypt.

10.21608/esctj.2022.179739.1023

Abstract

Background: Hyperglycemia associated with the diabetes mellitus (DM) commonly results in structural abnormalities and hyposalivation in the parotid glands. An antioxidant action in a potent multivitamin called Antox (ANX) aids in reducing oxidative stress. Aim of study: The current study examined the role of ANX in preventing complications from diabetes in the parotid gland, as well as its potential mechanisms. Material and methods: 36 rats were randomly divided into six groups; each group contained six rats .Group 1 (Negative (-ve) control): the rats received only regular food and water.Group 2 (vehicle group): rats received a single i.p. dose of citrate buffer as a vehicle. Group 3: rats were given ANX by oral gavage with a polyethylene canula 0.5 mm in a volume not to exceed 0.3 ml/100 gm at a dose of 10 mg/kg/day. Group 4 (Diabetic/ DM): rats received a single i.p. dose of 50 mg/kg of STZ dissolved in citrate buffer. Group 5 (DM+ Insulin): rats received a single i.p. dose of 50 mg/kg of STZ dissolved in citrate buffer and insulin (Mixtard 30/70; Novo Nordisk) 1 U/100 gm once daily subcutaneous (S.C.). Group 6 (DM+ Insulin + ANX): rats received a single i.p. dose of 50 mg/kg of STZ and insulin 1 U/100 gm /day/S.C. then received ANX orally in a dose of 10 mg/kg/day. All medications were given for 4 weeks. Results: Remarkably, co-administration of ANX with insulin significantly reduced (MDA) levels and increased expression of (8-OHdG) while enhancing the activities of (SOD), (GPX), and (TAC).

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